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For many years, AS/axSpA was considered to be a predominantly male disease but a relatively recent study from Switzerland, shows that the male to female ratio has declined from 2.57:1 in 1980 to 1.03:1 by the end of 2016

doi: 10.1038/s41388-020-01395-9 5 WarburgOWindFNegeleinE

The fluorescence intensity of each well was measured at Ex276 nm/Em545 nm using a Synergy H1 Microplate Reader (Bio Tek, Winooski, USA)

Anal Biochem 597:113638113646 Nonaka M, Tanaka H, Okiyama A, Motoki M, Ando H, Umeda K, Matsuura A (1989) Polymerization of several proteins by Ca2-independent transglutaminase derived from microorganisms